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low binding 384 well plate  (Eppendorf AG)


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    Structured Review

    Eppendorf AG low binding 384 well plate
    Low Binding 384 Well Plate, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 201118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/low+binding+plate+eppendorf/Eppendorf/bio_rxiv__64898__2026__04__26__720932-197-10-13
    Average 99 stars, based on 201118 article reviews
    low binding 384 well plate - by Bioz Stars, 2026-10
    99/100 stars

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    Article Title: A framework for ultra-low-input spatial tissue proteomics.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Recombinant Anti-Sodium Potassium ATPase antibody [EP1845Y] Abcam Cat#ab76020; RRID: AB_1310695 Recombinant Alexa Fluor 647 Anti-CD3D antibody [EP4426] Abcam Cat#ab198937; RRID: AB_2889190 CD20 Monoclonal Antibody (L26), Alexa Fluor 488, eBioscience Thermo Fisher Scientific Cat# 53-0202-80, RRID: AB_10734357 Pan Cytokeratin Monoclonal Antibody (AE1/AE3), eFluor 570, eBioscience Thermo Fisher Scientific Cat# 41-9003-80, RRID: AB_11217482 Biological samples Murine liver FFPE tissue fromC57BL/6mice The Jackson Laboratory https://www.jax.org/strain/000664 Human tonsil FFPE tissue Institute of Pathology at Charite University Hospital https://pathologie-ccm.charite.de/ Chemicals, peptides, and recombinant proteins n-Dodecyl-beta-Maltoside (DDM) Sigma-Aldrich Cat# D4641-500MG Endoproteinase Lys-C Promega Cat# VA1170 Proteomics grade modified trypsin Promega Cat# V5117 Tris(2-carboxyethyl)phosphine hydrochloride Sigma-Aldrich Cat# C4706-2G Acetonitrile (ACN) HPLC-grade VWR Cat# 83640.290 Isopropanol (ISO) Sigma-Aldrich Cat# 1070222511 Triethylammonium bicarbonate pH 8.5 (TEAB) Merck T7408-100ML Formic acid Merck Millipore Cat# 1.00264.1000 Trifluoroacetic acid Sigma-Aldrich Cat# 96924-250ML-F 2-chloroacetamide Sigma-Aldrich Cat# C0267-100G EnVision FLEX Target Retrieval Solution High pH (50X) Agilent Dako Cat# K8004 Microscopy Neo-Clear Sigma-Aldrich Cat# 1.09843.5000 Odyssey Blocking Buffer LI-COR Biosciences Cat# 927-70001 Prolong Diamond antifade mounting medium Invitrogen Cat# P36961 Aqua Poly Mount Polysciences Europe GmbH Cat# 18606-20 Pepsin solution for antigen retrieval Agilent Dako Cat# S3002 Deposited data Mass spectrometric raw files This paper ProteomeXchange: PXD042367 List of hepatocyte specific markers (Azimifar et al.22) 10.1016/j.cmet.2014.11.002 1-s2.0-S1550413114004999-mmc5.xlsx Software and algorithms DIA-NN version 1.8.1 Demichev et al.28 https://github.com/vdemichev/DiaNN R version 4.2.2 The R Project for Statistical Computing https://www.r-project.org/ Perseus version 1.6.15.0 Tyanova et al.50 https://maxquant.net/perseus/ Leica Laser Microdissection software version 8.3.0.08259 Leica Microsystems https://www.leica-microsystems.com/ products/microscope-software/p/leicalmd-software/ (Continued on next page) Cell Systems 14, 1002–1014.e1–e5, November 15, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Zeiss ZEN version 3.7 Carl Zeiss AG https://www.zeiss.com/microscopy/de/ produkte/software/zeiss-zen.html LAS X software version 3.7.524914 Leica Microsystems https://www.leica-microsystems.com/ products/microscope-software/p/leicalas-x-ls/ Qupath version 0.4.3 Bankhead et al.39 https://qupath.github.io/ Biological Image Analysis Software (BIAS) Single Cell Technologies https://single-cell-technologies.com/bias- 2/BioStudies Archive accession number SBSST820 Bruker Compass Data Analysis Software version 6.0 Bruker Daltonik GmbH https://www.bruker.com/en/products-and- solutions/mass-spectrometry/mssoftware.html Qupath_to_LMD function: Contour export from Qupath to the Leica LMD7 This paper https://doi.org/10.5281/zenodo.8414787 Other C18 Evotips (EV2013, Evotip Pure, Evosep) Evosep Biosystems https://www.evosep.com/evotip/ 96-well plate Thermo Fisher Scientific https://www.thermofisher.com/order/ catalog/product/de/en/AB1300 384-well low-binding plate Eppendorf Cat# 0030129547 Super PAP-pen liquid blocker mini Science Services Cat# N71312-N Cover glass Corning Cat# CLS2980223, #1.5 PPS frame slides Leica Cat# 11600294 PEN glass slides Carl Zeiss Cat# 15350731 ..

    Single Cell:

    Article Title: A framework for ultra-low-input spatial tissue proteomics.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Recombinant Anti-Sodium Potassium ATPase antibody [EP1845Y] Abcam Cat#ab76020; RRID: AB_1310695 Recombinant Alexa Fluor 647 Anti-CD3D antibody [EP4426] Abcam Cat#ab198937; RRID: AB_2889190 CD20 Monoclonal Antibody (L26), Alexa Fluor 488, eBioscience Thermo Fisher Scientific Cat# 53-0202-80, RRID: AB_10734357 Pan Cytokeratin Monoclonal Antibody (AE1/AE3), eFluor 570, eBioscience Thermo Fisher Scientific Cat# 41-9003-80, RRID: AB_11217482 Biological samples Murine liver FFPE tissue fromC57BL/6mice The Jackson Laboratory https://www.jax.org/strain/000664 Human tonsil FFPE tissue Institute of Pathology at Charite University Hospital https://pathologie-ccm.charite.de/ Chemicals, peptides, and recombinant proteins n-Dodecyl-beta-Maltoside (DDM) Sigma-Aldrich Cat# D4641-500MG Endoproteinase Lys-C Promega Cat# VA1170 Proteomics grade modified trypsin Promega Cat# V5117 Tris(2-carboxyethyl)phosphine hydrochloride Sigma-Aldrich Cat# C4706-2G Acetonitrile (ACN) HPLC-grade VWR Cat# 83640.290 Isopropanol (ISO) Sigma-Aldrich Cat# 1070222511 Triethylammonium bicarbonate pH 8.5 (TEAB) Merck T7408-100ML Formic acid Merck Millipore Cat# 1.00264.1000 Trifluoroacetic acid Sigma-Aldrich Cat# 96924-250ML-F 2-chloroacetamide Sigma-Aldrich Cat# C0267-100G EnVision FLEX Target Retrieval Solution High pH (50X) Agilent Dako Cat# K8004 Microscopy Neo-Clear Sigma-Aldrich Cat# 1.09843.5000 Odyssey Blocking Buffer LI-COR Biosciences Cat# 927-70001 Prolong Diamond antifade mounting medium Invitrogen Cat# P36961 Aqua Poly Mount Polysciences Europe GmbH Cat# 18606-20 Pepsin solution for antigen retrieval Agilent Dako Cat# S3002 Deposited data Mass spectrometric raw files This paper ProteomeXchange: PXD042367 List of hepatocyte specific markers (Azimifar et al.22) 10.1016/j.cmet.2014.11.002 1-s2.0-S1550413114004999-mmc5.xlsx Software and algorithms DIA-NN version 1.8.1 Demichev et al.28 https://github.com/vdemichev/DiaNN R version 4.2.2 The R Project for Statistical Computing https://www.r-project.org/ Perseus version 1.6.15.0 Tyanova et al.50 https://maxquant.net/perseus/ Leica Laser Microdissection software version 8.3.0.08259 Leica Microsystems https://www.leica-microsystems.com/ products/microscope-software/p/leicalmd-software/ (Continued on next page) Cell Systems 14, 1002–1014.e1–e5, November 15, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Zeiss ZEN version 3.7 Carl Zeiss AG https://www.zeiss.com/microscopy/de/ produkte/software/zeiss-zen.html LAS X software version 3.7.524914 Leica Microsystems https://www.leica-microsystems.com/ products/microscope-software/p/leicalas-x-ls/ Qupath version 0.4.3 Bankhead et al.39 https://qupath.github.io/ Biological Image Analysis Software (BIAS) Single Cell Technologies https://single-cell-technologies.com/bias- 2/BioStudies Archive accession number SBSST820 Bruker Compass Data Analysis Software version 6.0 Bruker Daltonik GmbH https://www.bruker.com/en/products-and- solutions/mass-spectrometry/mssoftware.html Qupath_to_LMD function: Contour export from Qupath to the Leica LMD7 This paper https://doi.org/10.5281/zenodo.8414787 Other C18 Evotips (EV2013, Evotip Pure, Evosep) Evosep Biosystems https://www.evosep.com/evotip/ 96-well plate Thermo Fisher Scientific https://www.thermofisher.com/order/ catalog/product/de/en/AB1300 384-well low-binding plate Eppendorf Cat# 0030129547 Super PAP-pen liquid blocker mini Science Services Cat# N71312-N Cover glass Corning Cat# CLS2980223, #1.5 PPS frame slides Leica Cat# 11600294 PEN glass slides Carl Zeiss Cat# 15350731 ..



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    Eppendorf AG dna low binding pcr plate
    Quantification of residual plasmid <t>DNA</t> in individual vaccine batches, expressed as nanograms per dose, based on simplex quantitative <t>PCR</t> (qPCR) assays. A DNA fragments derived from the region encoding the SARS-CoV-2 spike protein (SPIKE region). B DNA fragments derived from the region encompassing the bacterial origin of replication (ORI region). C DNA fragments derived from the region encoding kanamycin resistance in Comirnaty (KAN 1C) and Spikevax vaccine (KAN 1S). D Line display of all qPCR results of SPIKE, ORI and KAN assays to show consistent profiles of the batches. Each bar represents the mean value of triplicate reactions using optimised primer/probe sets. The dashed red line indicates the regulatory threshold of 10 ng/dose for residual DNA content. Unexpired vaccine batches are marked with an asterisk.
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    Quantification of residual plasmid DNA in individual vaccine batches, expressed as nanograms per dose, based on simplex quantitative PCR (qPCR) assays. A DNA fragments derived from the region encoding the SARS-CoV-2 spike protein (SPIKE region). B DNA fragments derived from the region encompassing the bacterial origin of replication (ORI region). C DNA fragments derived from the region encoding kanamycin resistance in Comirnaty (KAN 1C) and Spikevax vaccine (KAN 1S). D Line display of all qPCR results of SPIKE, ORI and KAN assays to show consistent profiles of the batches. Each bar represents the mean value of triplicate reactions using optimised primer/probe sets. The dashed red line indicates the regulatory threshold of 10 ng/dose for residual DNA content. Unexpired vaccine batches are marked with an asterisk.

    Journal: NPJ Vaccines

    Article Title: Systematic analysis of COVID-19 mRNA vaccines using four orthogonal approaches demonstrates no excessive DNA impurities

    doi: 10.1038/s41541-025-01304-9

    Figure Lengend Snippet: Quantification of residual plasmid DNA in individual vaccine batches, expressed as nanograms per dose, based on simplex quantitative PCR (qPCR) assays. A DNA fragments derived from the region encoding the SARS-CoV-2 spike protein (SPIKE region). B DNA fragments derived from the region encompassing the bacterial origin of replication (ORI region). C DNA fragments derived from the region encoding kanamycin resistance in Comirnaty (KAN 1C) and Spikevax vaccine (KAN 1S). D Line display of all qPCR results of SPIKE, ORI and KAN assays to show consistent profiles of the batches. Each bar represents the mean value of triplicate reactions using optimised primer/probe sets. The dashed red line indicates the regulatory threshold of 10 ng/dose for residual DNA content. Unexpired vaccine batches are marked with an asterisk.

    Article Snippet: In detail, a 30 μl aliquot of isolated DNA was transferred to a DNA low-binding PCR plate (LoBind, Eppendorf).

    Techniques: Plasmid Preparation, Real-time Polymerase Chain Reaction, Derivative Assay