low binding 384 well plate (Eppendorf AG)
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Low Binding 384 Well Plate, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 201118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 201118 article reviews
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Software:Article Title: A framework for ultra-low-input spatial tissue proteomics. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Recombinant Anti-Sodium Potassium ATPase antibody [EP1845Y] Abcam Cat#ab76020; RRID: AB_1310695 Recombinant Alexa Fluor 647 Anti-CD3D antibody [EP4426] Abcam Cat#ab198937; RRID: AB_2889190 CD20 Monoclonal Antibody (L26), Alexa Fluor 488, eBioscience Thermo Fisher Scientific Cat# 53-0202-80, RRID: AB_10734357 Pan Cytokeratin Monoclonal Antibody (AE1/AE3), eFluor 570, eBioscience Thermo Fisher Scientific Cat# 41-9003-80, RRID: AB_11217482 Biological samples Murine liver FFPE tissue fromC57BL/6mice The Jackson Laboratory https://www.jax.org/strain/000664 Human tonsil FFPE tissue Institute of Pathology at Charite University Hospital https://pathologie-ccm.charite.de/ Chemicals, peptides, and recombinant proteins n-Dodecyl-beta-Maltoside (DDM) Sigma-Aldrich Cat# D4641-500MG Endoproteinase Lys-C Promega Cat# VA1170 Proteomics grade modified trypsin Promega Cat# V5117 Tris(2-carboxyethyl)phosphine hydrochloride Sigma-Aldrich Cat# C4706-2G Acetonitrile (ACN) HPLC-grade VWR Cat# 83640.290 Isopropanol (ISO) Sigma-Aldrich Cat# 1070222511 Triethylammonium bicarbonate pH 8.5 (TEAB) Merck T7408-100ML Formic acid Merck Millipore Cat# 1.00264.1000 Trifluoroacetic acid Sigma-Aldrich Cat# 96924-250ML-F 2-chloroacetamide Sigma-Aldrich Cat# C0267-100G EnVision FLEX Target Retrieval Solution High pH (50X) Agilent Dako Cat# K8004 Microscopy Neo-Clear Sigma-Aldrich Cat# 1.09843.5000 Odyssey Blocking Buffer LI-COR Biosciences Cat# 927-70001 Prolong Diamond antifade mounting medium Invitrogen Cat# P36961 Aqua Poly Mount Polysciences Europe GmbH Cat# 18606-20 Pepsin solution for antigen retrieval Agilent Dako Cat# S3002 Deposited data Mass spectrometric raw files This paper ProteomeXchange: PXD042367 List of hepatocyte specific markers (Azimifar et al.22) 10.1016/j.cmet.2014.11.002 1-s2.0-S1550413114004999-mmc5.xlsx Software and algorithms DIA-NN version 1.8.1 Demichev et al.28 https://github.com/vdemichev/DiaNN R version 4.2.2 The R Project for Statistical Computing https://www.r-project.org/ Perseus version 1.6.15.0 Tyanova et al.50 https://maxquant.net/perseus/ Leica Laser Microdissection software version 8.3.0.08259 Leica Microsystems https://www.leica-microsystems.com/ products/microscope-software/p/leicalmd-software/ (Continued on next page) Cell Systems 14, 1002–1014.e1–e5, November 15, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Zeiss ZEN version 3.7 Carl Zeiss AG https://www.zeiss.com/microscopy/de/ produkte/software/zeiss-zen.html LAS X software version 3.7.524914 Leica Microsystems https://www.leica-microsystems.com/ products/microscope-software/p/leicalas-x-ls/ Qupath version 0.4.3 Bankhead et al.39 https://qupath.github.io/ Biological Image Analysis Software (BIAS) Single Cell Technologies https://single-cell-technologies.com/bias- 2/BioStudies Archive accession number SBSST820 Bruker Compass Data Analysis Software version 6.0 Bruker Daltonik GmbH https://www.bruker.com/en/products-and- solutions/mass-spectrometry/mssoftware.html Qupath_to_LMD function: Contour export from Qupath to the Leica LMD7 This paper https://doi.org/10.5281/zenodo.8414787 Other C18 Evotips (EV2013, Evotip Pure, Evosep) Evosep Biosystems https://www.evosep.com/evotip/ 96-well plate Thermo Fisher Scientific https://www.thermofisher.com/order/ catalog/product/de/en/AB1300 384-well Single Cell:Article Title: A framework for ultra-low-input spatial tissue proteomics. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Recombinant Anti-Sodium Potassium ATPase antibody [EP1845Y] Abcam Cat#ab76020; RRID: AB_1310695 Recombinant Alexa Fluor 647 Anti-CD3D antibody [EP4426] Abcam Cat#ab198937; RRID: AB_2889190 CD20 Monoclonal Antibody (L26), Alexa Fluor 488, eBioscience Thermo Fisher Scientific Cat# 53-0202-80, RRID: AB_10734357 Pan Cytokeratin Monoclonal Antibody (AE1/AE3), eFluor 570, eBioscience Thermo Fisher Scientific Cat# 41-9003-80, RRID: AB_11217482 Biological samples Murine liver FFPE tissue fromC57BL/6mice The Jackson Laboratory https://www.jax.org/strain/000664 Human tonsil FFPE tissue Institute of Pathology at Charite University Hospital https://pathologie-ccm.charite.de/ Chemicals, peptides, and recombinant proteins n-Dodecyl-beta-Maltoside (DDM) Sigma-Aldrich Cat# D4641-500MG Endoproteinase Lys-C Promega Cat# VA1170 Proteomics grade modified trypsin Promega Cat# V5117 Tris(2-carboxyethyl)phosphine hydrochloride Sigma-Aldrich Cat# C4706-2G Acetonitrile (ACN) HPLC-grade VWR Cat# 83640.290 Isopropanol (ISO) Sigma-Aldrich Cat# 1070222511 Triethylammonium bicarbonate pH 8.5 (TEAB) Merck T7408-100ML Formic acid Merck Millipore Cat# 1.00264.1000 Trifluoroacetic acid Sigma-Aldrich Cat# 96924-250ML-F 2-chloroacetamide Sigma-Aldrich Cat# C0267-100G EnVision FLEX Target Retrieval Solution High pH (50X) Agilent Dako Cat# K8004 Microscopy Neo-Clear Sigma-Aldrich Cat# 1.09843.5000 Odyssey Blocking Buffer LI-COR Biosciences Cat# 927-70001 Prolong Diamond antifade mounting medium Invitrogen Cat# P36961 Aqua Poly Mount Polysciences Europe GmbH Cat# 18606-20 Pepsin solution for antigen retrieval Agilent Dako Cat# S3002 Deposited data Mass spectrometric raw files This paper ProteomeXchange: PXD042367 List of hepatocyte specific markers (Azimifar et al.22) 10.1016/j.cmet.2014.11.002 1-s2.0-S1550413114004999-mmc5.xlsx Software and algorithms DIA-NN version 1.8.1 Demichev et al.28 https://github.com/vdemichev/DiaNN R version 4.2.2 The R Project for Statistical Computing https://www.r-project.org/ Perseus version 1.6.15.0 Tyanova et al.50 https://maxquant.net/perseus/ Leica Laser Microdissection software version 8.3.0.08259 Leica Microsystems https://www.leica-microsystems.com/ products/microscope-software/p/leicalmd-software/ (Continued on next page) Cell Systems 14, 1002–1014.e1–e5, November 15, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Zeiss ZEN version 3.7 Carl Zeiss AG https://www.zeiss.com/microscopy/de/ produkte/software/zeiss-zen.html LAS X software version 3.7.524914 Leica Microsystems https://www.leica-microsystems.com/ products/microscope-software/p/leicalas-x-ls/ Qupath version 0.4.3 Bankhead et al.39 https://qupath.github.io/ Biological Image Analysis Software (BIAS) Single Cell Technologies https://single-cell-technologies.com/bias- 2/BioStudies Archive accession number SBSST820 Bruker Compass Data Analysis Software version 6.0 Bruker Daltonik GmbH https://www.bruker.com/en/products-and- solutions/mass-spectrometry/mssoftware.html Qupath_to_LMD function: Contour export from Qupath to the Leica LMD7 This paper https://doi.org/10.5281/zenodo.8414787 Other C18 Evotips (EV2013, Evotip Pure, Evosep) Evosep Biosystems https://www.evosep.com/evotip/ 96-well plate Thermo Fisher Scientific https://www.thermofisher.com/order/ catalog/product/de/en/AB1300 384-well |
